When it comes to next-generation sequencing, the Illumina MiSeq 2 system is considered a top system to have in any lab. This is a very compact and small footprint type of sequencing system, and it is designed to be user-friendly and easy to integrate and incorporate into an existing lab or to be used in any type of research facility.
The advantage in the use of the Illumina MiSeq 2 next-generation sequencing technology is really the speed at which sequencing can occur. Rather than the much slower Sanger sequencing methods, next-generation sequencing provides rapid results in just hours rather than taking days to generate a large sample. In fact, timing is dramatically reduced to allow for prepping in about 90 minutes for sequencing libraries and amplification, sequencing, and base calling completed in less than six hours.
The Process
All Illumina sequencer models use sequencing by synthesis or SBS technology. This is one of the most used technologies in labs across the world, offering reliable, accurate and extremely cost-effective analysis for a wide range of different applications.
The automation with the Illumina MiSeq 2 is another important part of the process. Without the need for human management of the sequencing process, there is a dramatic reduction in errors and a much higher rate of accuracy and precision.
Controlling the system is done through the software that is loaded in the MiSeq. All controls are available through the onboard touch screen, which allows for easy setup and configuration of the system. Users can even view on-screen tutorials if there is any question as to the exact process required.
With a variety of different features including reagents that just need to be plugged into the equipment and full RDIF tracking for accurate record keeping, this is a significant addition to any lab.