Synaptophysin recognizes proteins with a molecular weight of 38kDa. It can help you label normal neuroendocrine cells of the carotid body, human adrenal medulla, skin, thyroid, pituitary gland, pancreas, lung, and gastrointestinal mucosa, as well as others. It can also label neurons in the spinal cord, retina, and brain. The anti-antibody of the same name can also react with neuroendocrine neoplasms of the epithelial and neural types, including ganglioneuroblastomas, neuroblastomas, pheochromocytomas, chromaffin, non-chromaffin paragangliomas, and ganglioneuromas. It is designed for research purposes and has no available clone. The immunogen is the synthetic peptide of the human synaptophysin. The isotype is the Rabbit IgG, and it has no determine epitope.
Synaptophysin can be used with Immunohistochemistry applications. The procedure requires that you use Formalin-fixed or paraffin-embedded tissues as specimens. You should use xylene, a xylene alternative, or graded alcohols for deparaffinized slides. In most cases, you will be required to dilute the product using a ratio of one to 300. However, please note that methods and protocols can vary, meaning the dilution rate may be different. If you do require the traditional diluted product, you can find pre-diluted formulas available.
When it is time to retrieve the antigen, you will need to boil the tissue section in a 10mM citrate buffer with a pH of 6.0. This must be done for 10 minutes, and then you should allow it to cool to room temperature for at least 20 minutes. Incubation periods are 10 minutes at room temperature, as well. It is important to rinse the slides with a PBS/0.05% Tween between steps. The positive control is the pheochromocytoma or the pancreas with cellular localizations occurring in the cytoplasm.
Synaptophysin is an antibody that can help with a variety of lab testing needs. Visit Spring Bioscience now to learn more.